In two years of observation, (spring 2006, autumn 2006, spring 2007 and autumn 2007) were being screened for parasites in total 495 faecal samples coming out of three farms from Ceske Budejovice (285 samples of sucking pigs not older then 28 days and 174 samples of piglets not older then 8 weeks). The method used to examine those samples was a flotation-concentrating method (Sheather{\crq}s carbohydrate fusion) and in the year of 2007 was also used a specific aniline-carbol-methyl violet staining method to detect the Cryptosporidium spp. followed by positive molecular characterized (direct sequencing of partial SSU rRNA partial genes and PCR-RFLP at the SSU rRNA). In screened samples were mainly detected parasites named Cryptosporidium spp., found in 4,1% of faecal samples in 2006 and in 2007 in 32,8% faecal samples, out of which 14,4% was found in pre-weaned piglets samples and 26,4% in post-weaned piglets. Based on genotyping provided on positive samples out of the year 2007, using method of sequensing analysis SSU rRNA, was the occurence of C. suis, Cryptosporidium pig genotype II aC. Muris described. High prevalence of Isosporou suis 13,9 % (64/459) was also detected with its appearance, in particular, in pre-weaned piglets 21,4 % (61/285). Further on, some of other identified group was Eimerie spp. 5,7 % (26/459) infecting, in the main, post-weaned piglets 10,9% (19/174) and Giardia intestinalis 2,4 % (10/459). Most of the samples mentioned occured in conditioned faeces and there is no seasonal relationship to the parasital occurance.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:51442 |
| Date | January 2009 |
| Creators | KOTILOVÁ, Jiřina |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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