This thesis reports the development of Comparative Genomic Fingerprinting
(CGF), a rapid genotyping method for Campylobacter jejuni that assesses the
conservation status of 20 genes previously described as having high intraspecies
variability based on comparative genomics studies. This novel method for genotyping C.
jejuni, CGF was validated two-fold. First, by comparison to flaA restriction fragment
length polymorphism analysis, and second a subset of isolates was validated using two
higher resolution CGF assays assessing 35 and 119 genes. CGF was then tested in a
molecular epidemiological study of C. jejuni isolated from environmental, animal and
human clinical samples from southern Alberta. Reservoirs of infection, subtypes
associated with higher incidence of human infection, and the persistence of prevalent
subtypes in animal/environmental reservoirs were identified. This thesis demonstrates
that CGF analysis is robust and can be used to rapidly assess genetic similarity of C.
jejuni isolates and to detect epidemiologically relevant clonal groups. / xii, 184 leaves : ill. ; 29 cm
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:ALU.w.uleth.ca/dspace#10133/2485 |
| Date | January 2010 |
| Creators | Ross, Susan, University of Lethbridge. Faculty of Arts and Science |
| Contributors | Gannon, Victor, Thomas, James |
| Publisher | Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2010, Arts and Science, Department of Biological Sciences |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
| Relation | Thesis (University of Lethbridge. Faculty of Arts and Science) |
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