This master's thesis is focused on cytogenetic analysis and karyotype variability of the Nemastomatidae family. This family comprises morphologically uniform harvestmen of small sizes, with low mobility, and with center of distribution in Europe. Karyotype differences could play an important role for detection of cryptic diversity in this family. The karyotype analysis is focused mainly on Alpine and Pyrenean endemic species but also on other taxons located in Central Europe. The goal was not only to identify the differences which occur between the specific genera and species, but also eventually between populations. For detection of the specific chromosomal alterations in evolution of the karyotype in Nemastomatidae the fluorescent in situ hybridization (FISH) was used, localizing the positions and amounts of gene clusters for 18S rRNA. From the results we can assume that the number of chromosomes in the family Nemastomatidae can range between 2n = 12- 30. Further it was found out that in Nemastomatidae the biarmed chromosomes are more prevalent, and that the species which have lower amounts of chromosomes contain chromosomes that noticeably differ in size (probably because of chromosomal fusions). These results indicate that with some morphologically uniform species, the knowledge of specific...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:380190 |
| Date | January 2018 |
| Creators | Alaverdyan, Argam |
| Contributors | Šťáhlavský, František, Symonová, Radka |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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