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Možnosti využití nanočástic různých kovů jako markerů pro imunoznačení ve skenovacím mikroskopu s autoemisní tryskou / The possibilities of using nanoparticles of various metals as the markers for immunocytochemical labelling in field emission scanning electron microscopy

Nowadays, electron microscopy is a widespread method used in many biological branches like medicine, physical science, mikrobiology or material technology. Scanning electron microscope is a type of an electron microscope that shows the sample surface by scanning it with a high-energy beam of electrons in a raster scan pattern. The signal of electrons reveals information about the sample such as morphology, chemical composition and structure and materials. Gold nanoparticles are widely used as a marker for immunolabeling in these days. The main point of this research was to find other nanoparticles, which can be used for multiple immunolabeling. The main task of this study is to conjugate these particles with atibodies, to use them for immunolocalisation on the convenient biological sample and to observe it in FESEM JEOL JSM-7401F.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:52434
Date January 2011
CreatorsEIBLOVÁ, Veronika
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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