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Previous issue date: 2017-12-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease of unknown origin that can cause inflammatory lesions in various organs. In recent years, platelet-related functions have been assigned in the pathophysiology of autoimmunity, inflammation, atherosclerosis and cancer immunology. The role of platelets as a marker of inflammation in SLE is an area of interest today. Mean platelet volume (MPV) has been the most studied index in autoimmune diseases recently, but the use of the immature platelet fraction (IPF) may bring new insights into the knowledge of platelet activity in SLE and we do not yet have a description of its behavior in SLE.
Objective: To quantify platelet indexes (platelet, MPV and IPF) in patients with active and inactive SLE compared to healthy blood bank controls. In parallel, correlate the indices with each other and with laboratory abnormalities of the disease.
Methods: Cross-sectional, controlled study. Adults with SLE according to the ACR 1997 criteria and healthy blood bank controls made up the groups. The platelet and MPV were obtained in automated counter XE-5000. The IPF was expressed as a percentage by flow cytometry, and cells with fluorescence for cytoplasmic RNA were positive.
Results: Forty-five patients with SLE (30 inactive) and 257 controls were evaluated. The median IPF was significantly higher in active SLE than in healthy controls (p = 0.032). There was a significant correlation between IPF and MVP in the lupus population (p <0.001, rs = 0.519) and controls (p <0.001, rs = 0.845). An inverse correlation between IPF and platelet counts was observed in active lupus (p = 0.025; rs = 0.575). There was no association between IPF and presence of anti-DNA.
Conclusion: The results of our data point to elevated IPF in patients with active SLE compared to healthy controls. The inverse correlation of IPF with plateletometry in active SLE is instigating finding and may stimulate further research. The role of IPF as an inflammatory marker in SLE demands future confirmation. / Introdu??o: O l?pus eritematoso sist?mico (LES) ? uma doen?a autoimune cr?nica de origem ainda desconhecida, podendo causar les?es inflamat?rias em diversos ?rg?os. Nos ?ltimos anos, t?m sido atribu?do ?s plaquetas fun??es relacionadas na fisiopatogenia da autoimunidade, inflama??o, aterosclerose e imunologia do c?ncer. O papel das plaquetas como marcador de inflama??o no LES constitui ?rea de interesse na atualidade. O volume plaquet?rio m?dio (VPM) tem sido o ?ndice mais estudado nas doen?as autoimunes recentemente, mas o uso do ?ndice de plaquetas imaturas (IPI) pode trazer novas luzes no conhecimento da atividade plaquet?ria no LES e ainda n?o temos descri??o do seu comportamento no LES.
Objetivo: Quantificar os ?ndices plaquet?rios (plaquetometria, VPM e IPI) em pacientes com LES ativo e inativo comparativamente a controles sadios de banco de sangue. Em paralelo, correlacionar os ?ndices entre si e com altera??es laboratoriais da doen?a.
M?todos: Estudo transversal, controlado. Adultos com LES de acordo com os crit?rios do ACR 1997 e controles sadios de banco de sangue compuseram os grupos. A plaquetometria e o VPM foram obtidos em contador automatizado XE-5000. O IPI foi expresso de forma percentual por citometria de fluxo, sendo positivas as c?lulas com fluoresc?ncia para RNA citopl?smico.
Resultados: Quarenta e cinco pacientes com LES (30 inativos) e 257 controles foram avaliados. A mediana do IPI foi significativamente maior no LES ativo do que em controles sadios (p=0,032). Houve correla??o significativa do IPI com o VPM na popula??o l?pica (p<0,001; rs=0,519) e nos controles (p<0,001, rs=0,845). Uma correla??o inversa entre IPI e plaquetometria foi observada nos l?picos ativos (p=0,025; rs=0,575). N?o houve associa??o entre IPI e presen?a de anti-DNA.
Conclus?o: Os resultados do nosso estudo apontam para IPI elevado em pacientes com LES ativo comparativamente a controles sadios. A correla??o inversa do IPI com plaquetometria no LES ativo ? um achado instigante e podeestimular novas pesquisas. O papel do IPI como marcador inflamat?rio no LES demanda confirma??o futura.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/8277 |
| Date | 28 December 2017 |
| Creators | Schmoeller, Deonilson Ghizoni |
| Contributors | Bodanese, Luiz Carlos, Staub, Henrique Luiz |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Escola de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 500, 500, 600, -224747486637135387, -969369452308786627, 2075167498588264571 |
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