A ribosomal system has been derived from <u>Cellvibrio gilus</u> which is active with respect to amino acid incorporation into hot trichloroacetic acid-insoluble material. Optimal incorporation systems showed a requirement for ribosomes, pH 5 enzyme protein, ATP plus ATP-generating system, magnesium ion, ammonium ion, and GTP. ¹⁴C-phenylalanine incorporation was stimulated by poly U in the presence of t-RNA which has been derived from this bacterium. The t-RNA had some activation effect on poly U-directed ¹⁴C-phenylalanine incorporation, whereas no effect on natural m-RNA primed ¹⁴C-leucine incorporation was observed. A complementary unlabeled amino acid mixture inhibited ¹⁴C-leucine incorporation. ¹⁴C-leucine incorporation was greatly inhibited by RBAase and puromycin, moderately inhibited by chloramphenicol, but not effected significantly by DNAase and actinomycin D. The optimum pH and temperature for ¹⁴C-leucine incorporation were 7.8 and 300° C, respectively. Polyamiaes (putrescine, spermine, and spermidine) had a slight stimulatory effect on poly U-directed.¹⁴C-phenylalanine incorporation, but no effect on ¹⁴C-Leucine incorporation. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/41212 |
| Date | 16 February 2010 |
| Creators | Ko, Thong-Sung |
| Contributors | Biochemistry and Nutrition, Barnett, Lewis B., Ackerman, C. J., Bunce, George Edwin, Campbell, T. Colin, King, Kendall W. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis, Text |
| Format | 93, 2 leaves, BTD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 34044396, LD5655.V855_1967.K57.pdf |
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