Return to search

Polygalacturonate lyase activity in Bacteroides from the human colon

In the course of studies on degradation of dietary fiber by the human colon bacteria, a number of Bacteroides strains from the human colon have been found to degrade polygalacturonic acid and/or pectin. Four of these strains were examined for presence of polygalacturonate lyase (PGAL). The Bacteroides strains studied synthesized inducible, cell-associated PGALs when the bacteria were grown on media containing polygalacturonic acid or on peanut cell wall (a prototype dietary fiber with a uronide content of 14.0%) as the sole carbon source. No PGAL activity could be detected in cultures grown on media containing glucose or D-galacturonate. The PGALs produced by the four Bacteroides strains had properties similar to other bacterial PGALs: An alkaline pH optima (8.5), stimulation of activity by calcium ions. complete inhibition by ethylenediaminetetraacetic acid (EDTA), preference for polygalacturonic acid as a substrate over pectin, and accumulation of unsaturated products which absorb at 235 nm. The Bacteroides PGALs catalyzed partial degradation of the uronides of peanut cell wall substrate, but at a slower rate compared to their action on polygalacturonic acid substrate. / M.S.

Identiferoai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/102592
Date January 1983
CreatorsChastain, Jane L.
ContributorsFood Science and Technology
PublisherVirginia Polytechnic Institute and State University
Source SetsVirginia Tech Theses and Dissertation
LanguageEnglish
Detected LanguageEnglish
TypeThesis, Text
Formatv, 60 leaves, application/pdf, application/pdf
RightsIn Copyright, http://rightsstatements.org/vocab/InC/1.0/
RelationOCLC# 10185572

Page generated in 0.0021 seconds