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The isolation and identification of lipoproteins associated with fatty acid synthesis in Penicillium chrysogenum

Penicillium chrysogenum was investigated to determine whether there are lipoproteins specifically associated with fatty acid synthesis and in what fraction of P. chrysogenum they are located. Radio-activity labeled free fatty acids and CoA thioester substrates were added to cell-free extracts and incubated. Samples of these incubation mixtures were analyzed for lipoprotein content with electrophoresis and for the presence of radioactivity with a radiochromatogram scanner and scintillation spectrometer.Label from fatty acyl thioester substrates migrated with protein fractions having alpha-2 or beta mobility showing the existence of lipoproteins associated with fatty acid metabolism in cell-free extracts of P. chryogenum. The percent distribution of radioactivity from the thioester incubations supported previous work in this laboratory on the desaturase system and suggested the presence of a long chain fatty acyl-CoA-ACP synthetase enzyme in Penicillium chrysogeum.

Identiferoai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/179875
Date January 1970
CreatorsPierce, Robert R.
ContributorsBennett, Alice S.
Source SetsBall State University
Detected LanguageEnglish
Format26 leaves ; 28 cm.
SourceVirtual Press

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