Return to search

An isoenzyme study in the Genus lotus (Fabaceae) /

An isoenzyme survey of several taxa within the genus Lotus was undertaken to provide markers for genetic research and to test hypotheses concerning the phylogenetic origin of Lotus corniculatus L. A preliminary study identified seven enzyme systems PGI, TPI, PGM, MDH, IDH, 6-PGDH and ME, that produced consistent clear phenotypes in L. uliginosus Schkuhr. Variation in phenotype with tissue type and stage of development suggested the presence of several isozyme zones in the phenotypes. Enzyme phenotype was constant for shoot tissue of plants older than six weeks. A second study examined recombination and segregation of isoenzyme phenotypes in interspecific hybrids, allo- and autopolyploids, and in L. corniculatus. Duplication and quadruplication of pgi2 loci in hybrids, amphidiploids, and in L. corniculatus was used as evidence that the latter is a segmental allotetraploid. A third study surveyed the occurrence of various isoenzyme alleles in L. alpinus Schleich., L. japonicus (Regel) Larsen, L. tenuis Waldst. & Kit., L. uliginosus and L. corniculatus. Lotus uliginosus had unique distinct alleles for several enzymes that did not occur in the other species. This evidence argues against the involvement of L. uliginosus in the origin of L. corniculatus.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75682
Date January 1987
CreatorsRaelson, John Verner
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Plant Science.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000660271, proquestno: AAINL45972, Theses scanned by UMI/ProQuest.

Page generated in 0.0017 seconds