A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc3688 |
| Date | 05 1900 |
| Creators | Coffee Castro-Zena, Pilar G. |
| Contributors | Root, Douglas D., Chapman, Kent D., Dickstein, Rebecca |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | Text |
| Rights | Public, Copyright, Coffee Castro-Zena, Pilar G., Copyright is held by the author, unless otherwise noted. All rights reserved. |
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