Bile acids are crucial for the uptake of dietary lipids and can shape the gut-microbiome
composition. This latter function is associated with the toxicity of bile acids and can be modulated
by bile acid modifying bacteria such as Eggerthella lenta, but the molecular details of the interaction
of bacteria depending on bile acid modifications are not well understood. In order to unravel the
molecular response to bile acids and their metabolites, we cultivated eight strains from a human
intestinal microbiome model alone and in co-culture with Eggerthella lenta in the presence of cholic
acid (CA) and deoxycholic acid (DCA). We observed growth inhibition of particularly gram-positive
strains such as Clostridium ramosum and the gram-variable Anaerostipes cacae by CA and DCA
stress. C. ramosum was alleviated through co-culturing with Eggerthella lenta. We approached effects
on the membrane by zeta potential and genotoxic and metabolic effects by (meta)proteomic and
metabolomic analyses. Co-culturing with Eggerthella lenta decreased both CA and DCA by the
formation of oxidized and epimerized bile acids. Eggerthella lenta also produces microbial bile salt
conjugates in a co-cultured species-specific manner. This study highlights how the interaction with
other bacteria can influence the functionality of bacteria.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:92011 |
| Date | 12 June 2024 |
| Creators | Jensen Pedersen, Kristian, Haange, Sven-Bastiaan, Žížalová, Katerina, Viehof, Alina, Clavel, Thomas, Lenicek, Martin, Engelmann, Beatrice, Wick, Lukas Y., Schaap, Frank G., Jehmlich, Nico, Rolle-Kampczyk, Ulrike, von Bergen, Martin |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2076-2607, 10.3390/microorganisms10102025 |
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