Abstract
The process of nitrification is highly dependent on the microbial activities and transformation, which is carried out by autotrophic nitrifiers in general, however some heterotrophic nitrifiers also can carry out the process. The diversity of autotrophic and heterotrophic ammonia-oxidizing bacteria in aquaculture ponds in Kaohsiung county was investigated. Ten heterotrophic bacteria were isolated. The nitrification ability and 16S rDNA sequences were determined. Seven of the strains had higher nitrification ability, five of them are belong to the genus of Pseudomonas, and the other two belong to Alcaligenes and Serratia, respectively. Both 16S rDNA and amoA gene sequences results showed that all autotrophic ammonia-oxidizing strains in this study belong to Nitrosomonas genus. From the data of 16S rDNA sequences, the strains isolated from Linyuan Shiang were distinct to the other two sites. Besides, amoA gene represents a very powerful molecular tool for analyzing ammonia-oxidizing bacteria communities due to its specificity and fine-scale resolution of closely related populations.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0628102-152221 |
| Date | 28 June 2002 |
| Creators | Peng, Ming-Chen |
| Contributors | Jong-Kang Liu, Chi-Hsin Hsu, Chan-Shing Lin |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628102-152221 |
| Rights | unrestricted, Copyright information available at source archive |
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