Enterococcus faecalis 710C is a lactic acid bacterium that produces two bacterocins, ent7A and ent7B. Both ent7A and ent7B have strong activity against gram-positive food pathogens including Listeria spp., Clostridium spp., vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Mass spectrometry analyses revealed that both ent7A (5201 Da) and ent7B (5207 Da) have formylated N-terminal methionine. The amino acid sequences, structural gene sequences of ent7 from nucleotide position 1-275 and immunity gene were determined. Circular dichroism data suggest that in aqueous solution ent7A and ent7B have 20 to 25% alpha-helical region. Addition of membrane-mimicking reagent (trifluoroethanol) did not significantly enhance the alpha-helical content in ent7A and ent7B. Chiral analysis by gas chromatography- mass spectrometry showed that the amino acid residues elucidated in ent7A and ent7B were all in L-configuration. / Food Science and Technology
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/902 |
Date | 06 1900 |
Creators | Liu, Xiaoji |
Contributors | McMullen, Lynn (Agricultural, Food and Nutritional Science), McMullen, Lynn (Agricultural, Food and Nutritional Science), Vederas, John (Chemistry), Michael, Gnzle (Agricultural, Food and Nutritional Science) |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Thesis |
Format | 497526 bytes, application/pdf |
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