The work presented in this thesis is concerned with the
effect of several antibiotics and quaternary ammonium
sanitizers upon growth of lactic acid bacteria. Section I
reports the purification of beta-lactamase from Lactococcus
cremoris PR-108, by ion exchange chromatography, using the
chromogenic substrate pyridine-2-azo-p-dimethylaniline
(PADAC) as the enzymatic indicator. Section II reports a
study of the influence of antibiotics on lactococcal growth,
where the effects of incubation time, culture dilution and the
use of seeded and spread agar plate techniques are
investigated. These studies were extended, in section III,
to include investigations of the effect of quaternary ammonium
base sanitizer (Ster-bac) on lactic starters. In addition,
this section describes an reverse phase high performance
liquid chromatography assay for the detection of quaternary
ammonium compounds in milk. / Graduation date: 1991
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/37622 |
| Date | 13 June 1990 |
| Creators | Valladao, Marilin |
| Contributors | Sandine, William E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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