Development of the vertebrate central nervous system is a complex process that relies on the accurate spatiotemporal distribution of signaling centers during embryogenesis. These signals provide cells with positional information, which is integrated via transcription factors and gene regulatory elements to generate a specific downstream gene expression profile that confers specific cellular functions. It is of interest to determine how cells acquire their unique spatiotemporal gene expression patterns. The wide variety of expression profiles established along the dorsoventral axis of the neural tube provides a great system to address this question. Recent advances in zebrafish transgenic technology, along with the phenomenon of a fish-specific genome duplication event, have been exploited here to provide an efficient way of identifying and characterizing gene regulatory elements. An identified neuronal-specific enhancer near the ladybird locus has been incorporated into a transgenic zebrafish strain driving fluorescent reporter protein expression in a subset of dorsal interneurons / Molecular Biology and Genetics
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/994 |
| Date | 06 1900 |
| Creators | Lukowski, Chris |
| Contributors | Waskiewicz, Andrew (Biological Sciences), Allison, William (Biological Sciences), Underhill, Alan (Medical Genetics), Pilgrim, David (Biological Sciences) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 33935282 bytes, application/pdf |
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