The mitochondnal genome was screened for genetic variation associated with production traits in White Leghorns. Analysis was done on an unselected randomly mated population of White Leghorn chickens. An AsnI restriction fragment length polymorphism (RFLP) was detected in the control region of the mitochondrial genome, a region responsible for transcription and replication. The RFLP segregated at a frequency of 0.76 for the AsnI (+) allele (presence of an AsnI restriction site). The AsnI RFLP was in linkage disequilibrium with a previously detected MspI RFLP in the NADH sub-unit IV of the mitochondrial genome for the same population of White Leghorns. Genotypes were denoted as follows: The first sign represented individuals that were positive (+) or negative (-) for the MspI restriction site, where as the second denoted whether individuals were positive or negative for the AsnI restriction site. Comparison of the three genotypic classes (+,+), (-,+) and (-,-) revealed that (-,-) individuals had a significantly higher body weight (5.5%) than individuals of the other genotypes. Dot blot analysis revealed no difference in the mitochondrial DNA copy number in peripheral blood cells between AsnI(-) or AsnI(+) individuals.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30380 |
| Date | January 1999 |
| Creators | Fourtounis, Dimitrios. |
| Contributors | Kuhnlein, Urs (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Animal Science.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001744379, proquestno: MQ64356, Theses scanned by UMI/ProQuest. |
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