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salerno_t_dr_botfmvz.pdf: 1479294 bytes, checksum: 37333e3500ad6a6f49d6aabf4f562cf3 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente estudo avaliou a qualidade microbiológica, as características físicoquímicas e a presença de resíduos de antimicrobianos em leite de mulheres, vacas e cabras, bem como investigou a multirresistência bacteriana à antimicrobianos e a detecção de genes e produção de toxinas em linhagens de Staphylococcus spp. Foram colhidas 240 amostras de leite de mulheres encaminhadas ao Banco de Leite Humano (BLH) de Botucatu, SP, 200 amostras de leite de vacas com mastite e 200 de vacas sem mastite. Iguais quantidades de amostras de leite foram colhidas de cabras com e sem mastite. Dentre os tetos amostrados de vacas e cabras com mastite, 85,50% e 97,50% respectivamente acusaram mastite subclínica no CMT. A mastite clínica foi observada em 14,50% dos tetos de vacas e 2,50% dos tetos de cabras amostrados. A presença de micro-organismos em leite de vacas e cabras sem mastite foi verificada em cerca de 25,00% das amostras de leite testadas, alertando para a presença de animais portadores de patógenos no rebanho. A acidez dornic do leite de mulheres revelou que 95,42% encontraram-se dentro dos limites aceitáveis pela Rede Nacional de BLH. O aporte calórico do leite humano (LH) apresentou ampla variação nos teores de creme, gordura e valor energético. A acidez dornic do leite de vacas e cabras com e sem mastite apresentaram ampla variação indicando que outros fatores, além da contaminação microbiana do leite, podem interferir nos índices de acidez titulável. Staphylococcus spp. e Enterobacter spp. foram os isolados mais frequentes em amostras de LH. Streptococcus spp., Staphylococcus spp. e Corynebacterium bovis foram os micro-organismos mais comumente... / The present study evaluated the microbiological quality, the characteristics physicist-chemistries and the presence of antimicrobials residues in milk of women, cows and goats, as well as investigated the bacterial multidrug resistance and the detection of genes and/or toxin production in Staphylococcus spp. strains. Was collected 240 milk samples from women referred to the Human Milk Bank (HMB) in Botucatu, 200 milk samples from cows with mastitis and 200 cows without mastitis. Equal amounts of milk samples were collected from goats with and without mastitis. Among the sampled cows and goats teats with mastitis, 85.50% and 97.50% respectively accused subclinical mastitis on CMT. Clinical mastitis was observed in 14.50% of cows teats and 2.50% of the sampled goats teats. The presence of microorganisms in milk from cows and goats without mastitis was found in about 25.00% of the milk samples tested, warning of the presence of pathogens from animals in the herd. Dornic acidity of woman’s milk revealed that 95.42% were within acceptable limits by the National Network of HMB. Calorie intake of human milk (HM) showed wide variation in the amounts of cream, fat and energy value. Dornic acidity of cows and goats milk with and without mastitis showed wide variation indicating that other factors in addition to microbial contamination of milk can interfere with acidity indices. Staphylococcus spp. and Enterobacter spp. were the most frequently isolated in samples of human milk. Streptococcus spp., Staphylococcus spp. and Corynebacterium bovis were the microorganisms most commonly isolated in milk cows with and without mastitis. In milk samples from goats with and without... (Complete abstract click electronic access below)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/106026 |
| Date | 02 September 2011 |
| Creators | Salerno, Tatiana [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Ribeiro, Márcio Garcia [UNESP], Pinto, José Paes de Almeida Nogueira [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 212 f. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1, -1 |
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