In a limited screen of monokaryons and dikaryons of various strains of Trametes versicolor, the monokaryons generally bleached hardwood and softwood kraft pulps more extensively than dikaryons, although there was great variation among both groups. A monokaryotic strain (52J) derived from a Paprican dikaryon (52P) by regenerating mycelium-derived protoplasts had better pulp bleaching ability than its dikaryotic parent. Unusually low extracellular laccase and manganese peroxidase activity levels were associated with reduced pulp bleaching. Mutants of strain 52J were isolated with various manganese peroxidase and laccase activities. Those with markedly reduced laccase and manganese peroxidase activity had impaired abilities to bleach, delignify, and produce methanol from hardwood kraft pulp, mineralize $ sp{14}$C-labelled synthetic lignins, reduce the colour of kraft mill bleach plant effluent, or had lost these abilities altogether. Dechlorination of kraft bleach plant effluent organochlorines was only slightly less in the mutants tried than in 52J. When purified manganese peroxidase was added back to mutant M49 of 52J, significant pulp bleaching was restored. Effluent decolorization also improved with added manganese peroxidase.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41516 |
| Date | January 1994 |
| Creators | Addleman, Katherine |
| Contributors | Archibald, Frederick (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001396210, proquestno: NN94571, Theses scanned by UMI/ProQuest. |
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