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Previous issue date: 2007 / Objetivo: Investigar a possivel participacao de celulas B-1 na inducao de tolerancia oral em camundongos submetidos a um regime de alimentacao continua com OVA. Metodos: Camundongos femeos das linhagens BALB/c e BALB/xid, entre 6 e 8 semanas, foram utilizados na formacao de 9 grupos experimentais, composto respectivamente pelos grupos de animais tratados previamente com OVA por via oral, (B/c-T e Bx-T); grupo de animais que nao receberam tratamento oral com OVA mas foram imunizados, par via s.c. com OVA emulsificada (1:1) em Adjuvante completo de Freund (B/c-I e B/x-I); grupo de animais que foram somente desafiados com OVA agregada (B/c-N e B/x-N); grupos B/x-recN, B/x-recT e B/x-recl compostos por camundongos BALB/Xid reconstituidos por transferencia adotiva com celulas B-1 do sobrenadante de cultura de celulas aderentes peritoneais de camundongos BALB/c naive; tolerizados e imunes, respectivamente. Esses animais receberam transferencia adotiva de celulas B-1 tres dias antes da imunizacao. A resposta foi avaliada por: a) reacao inflamatoria local; b) linfoproliferacao pela incorporacao de 3+[H]Timidina e c) determinacao por ELISA de imunoglobulinas anti-¬OVA das subclasses (IgG total, IgG1 e IgG2a). Resultados: Em camundongos BALB/c 0 tratamento oral com OVA previamente a imunizacao foi capaz de suprimir reacao local de hipersensibilidade e resposta celular muito embora esse efeito nao tenha sido observado em camundongos BALB/Xid submetidos ao mesmo tipo de tratamento. No entanto, quando camundongos BALB/Xid foram transferidos adotivamente com celulas de sobrenadante de cultura celular de peritoneo de camundongos BALB/c tolerizados,que esses animais desenvolveram supressao da reacao local de hipersensibilidade e supressao da resposta celular. Conclusao: Celulas B-1 da cavidade peritoneal de camundongos BALB/c doadores, submetidos ao tratamento oral com OVA, foram eficazes em promover a supressao da reacao de hipersensibilidade local e da resposta celular linfoproliferativa, mas nao a de producao de anticorpos, quando adotivamente transferidas para camundongos BALB/Xid, refratarios a inducao de tolerancia oral / Purpose: To investigate a possible participation of B-1 cells in inducing oral
tolerance in mice submitted to continuous feeding with OVA.
Methods: Female mice of the BALB/c and BALB/Xid lineages, aged 6 to 8 weeks,
were divided into 9 groups and submitted to the following conditions: previous oral
treatment with OVA (B/c-T and Bx-T); without oral treatment with OVA, but with
subcutaneous immunization with OVA emulsion (1:1) in complete Freund's adjuvant
(B/c-I and B/x-I); challenge with aggregated OVA only (B/c-N and B/x-N); adoptive
transfer (rebuilding), to BALB/Xid mice, of B-1 cells in culture supernatant with
peritoneal adherent cells from naïve BALB/c mice (B/x-recN, B/x-recT, and B/x-recI);
made tolerant and immune, respectively. These animals received adoptive transfer of
B-1 cells three days prior to immunization. The immune responses were evaluated by
means of: a) local inflammatory response; b) lymphoproliferation by incorporation of
3+[H]Thymidine and c) determination of anti-OVA immunoglobulin (subclasses total
IgG, IgG1, and IgG2a) by ELISA.
Results: Oral treatment with OVA prior to immunization suppressed the local
hypersensitivity reaction and cellular response in BALB/c mice, although this effect
was not observed in BALB/Xid mice in the same conditions. However, when
BALB/Xid mice were reconstituted with cultured cells from tolerized peritoneal
BALB/c mice, the suppression of local hypersensitivity and cellular response were
observed.
Conclusion: B-1 cells from BALB/c mice orally treated with OVA caused phenotypic
and functional changes and suppression of cellular response local and
linfoproliferative and local hypersensitivity reaction when adoptively transferred to
BALB/Xid mice refractory to induction of oral tolerance. / BV UNIFESP: Teses e dissertações
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unifesp.br:11600/23612 |
| Date | January 2007 |
| Creators | Gennaro, Luiz Antonio de [UNIFESP] |
| Contributors | Mariano, Mario [UNIFESP] |
| Publisher | Universidade Federal de São Paulo (UNIFESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 111 f. |
| Source | reponame:Repositório Institucional da UNIFESP, instname:Universidade Federal de São Paulo, instacron:UNIFESP |
| Rights | info:eu-repo/semantics/openAccess |
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