Induction of extracellular lipases from Pseudomonas fragi CRDA 037 and Geotrichum candidum was used to increase lipase production in a shorter period of time. Induction was performed using edibles oils such as olive oil, canola oil, fish oil and butter fat. Fermentation trials of the microorganisms in the selected media were done in order to optimize the production of lipases. Optimal lipase activity was obtained in the presence of butter fat (1%, v/v) for P. fragi which was cultivated at 15°C during 48 h, and fish oil (0.75%, v/v) for G. candidum incubated at 27°C for 56 h. The induced lipase extracts of P. fragi and G. candidum obtained after these fermentation trials were purified by 3.7 and 5.9-fold respectively using ultrafiltration, while the non-induced fractions were purified by 2.3 and 5.1-fold, respectively. Activities were evaluated using p-nitrophenyl esters such as p-nitrophenyl laurate and p-nitrophenyl palmitate. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30712 |
| Date | January 2000 |
| Creators | Ngom, Marie Odile. |
| Contributors | Kermasha, Selim (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Food Science and Agricultural Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001747405, proquestno: MQ64416, Theses scanned by UMI/ProQuest. |
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