Huian plasma high-density lipoprotein (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. Lipid and protein composition of the HDL isolated by this method was found to be similar to another HDL preparation isolated by ultracentrifugation. However, minor differences were noted, including a higher phospholipid and apoproteinE content and lower triglyceride content of the HDL isolated by column chromatography. Four subfraction of HDL were obtained following chromatography on an anion exchange column. The subfraction four had the highest esterified to free cholesterol ratio, the second highest phospholipid to unesterified cholesterol, and the lowest molecular weight. In addition it was consistently coincided with lecithin: cholesterol acyltransferase (LCAT) activity and found to be the best substrate for the enzyme.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc504553 |
| Date | 05 1900 |
| Creators | Jahani, Mehrnoosh |
| Contributors | Lacko, Andras G., Harris, Ben G., Masaracchia, Ruthann A., Thompson, Richard E. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | i, 65 leaves: ill., Text |
| Rights | Public, Jahani, Mehrnoosh, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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