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Effects of glycyrrhizic acid (GA) on aflatoxin B₁ (AFB₁) induced cytotoxicity.

by Chan Hoi Tak. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 129-137). / Abstracts in English and Chinese. / Acknowledgment --- p.i / Abstract --- p.ii / Abstract (Chinese Version) --- p.iv / Abbreviations --- p.vi / Contents --- p.viii / Chapter CHAPTER ONE --- INTRODUCTION --- p.1 / Chapter 1.1 --- Aflatoxins --- p.1 / Chapter 1.1.1 --- AFB1 Metabolism --- p.5 / Chapter 1.1.2 --- Bioactivation --- p.5 / Chapter 1.1.3 --- Detoxification --- p.8 / Chapter 1.1.4 --- Toxicity of AFB1 --- p.9 / Chapter 1.2 --- Licorice Plants --- p.11 / Chapter 1.2.1 --- Glycyrrhizic Acid (GA) --- p.13 / Chapter 1.2.2 --- Metabolism of GA --- p.16 / Chapter 1.2.3 --- Adverse Effects of GA --- p.19 / Chapter 1.3 --- Aim of Research --- p.19 / Chapter CHAPTER TWO --- MATERIALS AND METHODS --- p.21 / Chapter 2.1 --- Cell cultures --- p.21 / Chapter 2.1.1 --- Isolation of splenocytes --- p.21 / Chapter 2.1.2 --- Culture of cell lines --- p.22 / Chapter 2.1.3 --- Trypsinziation of cells --- p.22 / Chapter 2.2 --- Preparation of drugs --- p.23 / Chapter 2.2.1 --- Preparation of commercially available chemicals for cell culture --- p.23 / Chapter 2.2.2 --- Preparation of Licorice extracts for cell culture --- p.23 / Chapter 2.2.3 --- Preparation of chemicals for enzymatic reaction --- p.24 / Chapter 2.3 --- Cytotoxicity assay Methods --- p.24 / Chapter 2.3.1 --- MTT assay --- p.25 / Chapter 2.3.2 --- Neutral red assay --- p.25 / Chapter 2.4 --- Cytotoxicity Assay --- p.32 / Chapter 2.5 --- Chemoprotection assay --- p.32 / Chapter 2.5.1 --- Direct addition of drugs --- p.33 / Chapter 2.5.2 --- Pretreatment of cells --- p.33 / Chapter 2.5.3 --- Culture of cells with drugs --- p.34 / Chapter 2.6 --- Preparation of enzymes --- p.36 / Chapter 2.6.1 --- Preparation of cells --- p.36 / Chapter 2.6.2 --- Pretreatment of rat microsomes --- p.36 / Chapter 2.7 --- Bradford Assay --- p.37 / Chapter 2.8 --- Alkoxyresorufin O-Dealkylase assay --- p.39 / Chapter 2.8.1 --- Effects of AFB1 on P450 --- p.44 / Chapter 2.8.2 --- Effects of drugs on P450 --- p.44 / Chapter 2.9 --- Glutathione-S-transferase assay --- p.44 / Chapter 2.9.1 --- GST assay in rat microsomal fractions --- p.48 / Chapter 2.9.1.1 --- Effects of AFB1 on GST --- p.48 / Chapter 2.9.1.2 --- Effects of drugs on GST --- p.48 / Chapter 2.9.2 --- GST assay in crude fractions from cells --- p.48 / Chapter 2.9.2.1 --- Direct addition effects of drugs on GST --- p.48 / Chapter 2.9.2.2 --- Effects of drug-containing medium on GST --- p.49 / RESULTS / Chapter CHAPTER THREE --- CELL CULTURES --- p.50 / Chapter 3.1 --- Cytotoxicity assay --- p.51 / Chapter 3.1.1 --- Cytotoxicity of AFB1 --- p.51 / Chapter 3.1.1.1 --- Cytotoxicity of AFB1 to splenocytes --- p.51 / Chapter 3.1.1.2 --- Cytotoxicity of AFB1 to cell lines --- p.57 / Chapter 3.1.2 --- Cytotoxicity of drugs to cell lines --- p.62 / Chapter 3.2 --- Chemoprotection assay --- p.73 / Chapter 3.2.1 --- Direct addition of drugs --- p.73 / Chapter 3.2.2 --- Pretreatment of cells with drugs --- p.78 / Chapter 3.2.3 --- Culture of cells with drugs --- p.83 / Chapter CHAPTER FOUR --- ENZYMATIC ASSAYS --- p.87 / Chapter 4.1 --- Alkoxyresorufin O-dealkylase assay --- p.88 / Chapter 4.1.1 --- Effects of AFB1 on P450s --- p.88 / Chapter 4.1.2 --- Effects of GA on P450s --- p.92 / Chapter 4.1.3 --- Effects of EX on P450s --- p.95 / Chapter 4.2 --- Glutathione -S- transferase assay --- p.98 / Chapter 4.2.1 --- Effects of drugs on GST in cells --- p.100 / Chapter 4.2.2 --- Effects of AFB1 and drugs on GST from rats --- p.108 / Chapter 4.2.2.1 --- Effects of AFB1 on GST from rats --- p.108 / Chapter 4.2.2.2 --- Effects of drugs on GST from rats --- p.112 / Chapter CHAPTER FIVE --- DISCUSSION --- p.115 / Chapter 5.1 --- Cytotoxicity of AFB1 --- p.118 / Chapter 5.2 --- "Cytotoxicity of EX, GA and AA" --- p.119 / Chapter 5.3 --- Chemoprotection of GA and EX on AFB1 cytotoxicity --- p.121 / Chapter 5.4 --- Effects of GA and EX on enzymes involved in AFB1 metabolism --- p.123 / REFERENCES --- p.129

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322649
Date January 1999
ContributorsChan, Hoi Tak., Chinese University of Hong Kong Graduate School. Graduate Board on Environmental Science.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 137 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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