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ZBP-89 enhances Bak expression and causes apoptosis in hepatocellular carcinoma cells.

To, Ka Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (p. 115-120). / Abstracts in English and Chinese. / Acknowledgement --- p.i / Abstract --- p.ii / 中文摘要 --- p.vi / List of abbreviations --- p.ix / List of tables --- p.xii / List of figures --- p.xiii / Contents --- p.xvi / Chapter Chapter One: --- General introduction --- p.1 / Chapter 1.1 --- Background of Hepatocellular carcinoma (HCC) --- p.2 / Chapter 1.2.1 --- ZBP-89 structure and its expression in cancers --- p.3 / Chapter 1.2.2 --- Transcriptional regulation of ZBP-89 --- p.5 / Chapter 1.3.1 --- Apoptosis and necrosis --- p.6 / Chapter 1.3.2 --- Mechanisms of Apoptosis --- p.7 / Chapter 1.4 --- Bcl-2 family --- p.11 / Chapter 1.5 --- Regulation of p53 cancer cells --- p.12 / Chapter 1.6 --- The aim of the study --- p.13 / Chapter Chapter Two: --- Up-regulation of Bak expression by Ad-ZBP-89 induce apoptosis in human liver cancer cells --- p.15 / Chapter 2.1. --- Introduction --- p.16 / Chapter 2.2. --- Materials and methods --- p.18 / Chapter 2.2.1. --- Cell culture --- p.18 / Chapter 2.2.2. --- RT-PCR --- p.19 / Chapter 2.2.3. --- Western blotting --- p.21 / Chapter 2.2.4. --- Adenovirus infection and Cell viability assay --- p.24 / Chapter 2.2.5. --- Detection of apoptosis --- p.26 / Chapter 2.2.6. --- RNA interference --- p.27 / Chapter 2.2.7. --- Statistical analysis --- p.29 / Chapter 2.3. --- Results --- p.30 / Chapter 2.3.1. --- Endogenous expression of ZBP-89 and Bak of human liver cancer cells --- p.30 / Chapter 2.3.2. --- Effects of Ad-ZBP-89 on proliferation in HCC cell lines --- p.31 / Chapter 2.3.3. --- Effects of Ad-ZBP-89 on the expression of Bcl-2 family members --- p.34 / Chapter 2.3.4. --- ZBP-89 induced Bak expression and release of cytochrome c --- p.39 / Chapter 2.3.5. --- Effects of Ad-ZBP-89 on apoptosis rate in HCC cell lines --- p.41 / Chapter 2.3.6. --- Effects of ZBP-89 siRNA on expression of Bcl-2 family members and proliferation in HCC cell lines --- p.43 / Chapter 2.3.7. --- Effects of Bak siRNA and its combined effect with Ad-ZBP-89 on the expression of Bak and reduced apoptosis in HCC cell lines --- p.50 / Chapter 2.4. --- Discussion --- p.55 / Chapter Chapter Three: --- Identification of ZBP-89 protein as an apoptosis activator for a pro-apoptotic Bak gene promoter --- p.60 / Chapter 3.1. --- Introduction --- p.61 / Chapter 3.2. --- Materials and methods --- p.64 / Chapter 3.2.1. --- Cell lines and tissues --- p.64 / Chapter 3.2.2. --- Transient transfection and Luciferase activity assay --- p.64 / Chapter 3.2.3. --- pGL3-Bak-promoter vector construction --- p.67 / Chapter 3.2.4. --- "Preparation of mitochondrial, cytosolic and nuclear fractions" --- p.74 / Chapter 3.2.5. --- Electrophoretic mobility shift assay --- p.75 / Chapter 3.2.6. --- Overexpression of Bak --- p.76 / Chapter 3.2.7. --- RT-PCR and Western blot analysis on HCC tissues samples --- p.80 / Chapter 3.2.8. --- Statistical Analysis --- p.80 / Chapter 3.3. --- Results --- p.81 / Chapter 3.3.1. --- ZBP-89 activates Bak-luciferase promoter genes in HCC cells --- p.81 / Chapter 3.3.2. --- ZBP-89 activates shortened Bak-luc-promoter in PLC/PRF/5 and SK-Hep-1 cells --- p.82 / Chapter 3.3.3. --- ZBP-89 is a potential binding protein to the Bak promoter gene region -457/-407 --- p.85 / Chapter 3.3.4. --- The combined effects of Bak overexpression and Ad-ZBP-89 induce apoptosis in HCC cells --- p.89 / Chapter 3.3.5. --- The combined effects on Bak protein expression --- p.94 / Chapter 3.3.6. --- Bak expression in HCC tissues --- p.98 / Chapter 3.4. --- Discussion --- p.99 / Chapter Chapter Four: --- Conclusion and Future Perspectives --- p.104 / Chapter 4.1. --- Conclusion --- p.104 / Chapter 4.2. --- Future Perspectives --- p.112 / Reference --- p.113

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326653
Date January 2009
ContributorsTo, Ka Yan., Chinese University of Hong Kong Graduate School. Division of Surgery.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvii, 120 p. : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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