In tilapia , sex determination is controled by genetic and triggered by the environmental factors. Expressed sequence tags ( EST ) derived from the developing tilapia brain is cloned in our lab. The cDNA full length of the gene, F10A83 was cloned. In the present study, F10A83 is a gene with 1526 bp of cDNA sequence, and deduced 176 amino acids of protein sequence. F10A83 was overexpressed as a GFP fusion protein in mouse neuroblastoma Neuro-2a cells. F10A83 is abundantly distributed in the nucleus of Neuro-2a cell. The protein of F10A83 was expressed in the prokaryotic cell (BL21) and eukaryotic cell (neuro-2a), and purified using a simple purification process, inducing, isolation, and Ni-NTA affinity chromatography. The protein of F10A83 in both E. coli system and neuro-2a cell line expression has been established.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0905106-162229 |
| Date | 05 September 2006 |
| Creators | Huang, Sheng-hui |
| Contributors | Hong Yi-Ren, Ching-Lin Tasi, Hsiao-Sheng Liu, Li-Hsueh Wang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905106-162229 |
| Rights | not_available, Copyright information available at source archive |
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