Return to search

Protein expression of low temperature-inducible gene in Tilapia, Oreochromis mossambicus

In tilapia , sex determination is controled by genetic and triggered by the environmental factors. Expressed sequence tags ( EST ) derived from the developing tilapia brain is cloned in our lab. The cDNA full length of the gene, F10A83 was cloned. In the present study, F10A83 is a gene with 1526 bp of cDNA sequence, and deduced 176 amino acids of protein sequence. F10A83 was overexpressed as a GFP fusion protein in mouse neuroblastoma Neuro-2a cells. F10A83 is abundantly distributed in the nucleus of Neuro-2a cell. The protein of F10A83 was expressed in the prokaryotic cell (BL21) and eukaryotic cell (neuro-2a), and purified using a simple purification process, inducing, isolation, and Ni-NTA affinity chromatography. The protein of F10A83 in both E. coli system and neuro-2a cell line expression has been established.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0905106-162229
Date05 September 2006
CreatorsHuang, Sheng-hui
ContributorsHong Yi-Ren, Ching-Lin Tasi, Hsiao-Sheng Liu, Li-Hsueh Wang
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905106-162229
Rightsnot_available, Copyright information available at source archive

Page generated in 0.002 seconds