Prostaglandin F2α (PGF2α) is commonly given to female cattle intramuscularly (IM) for the synchronization of estrus. A novel site for administration of PGF2α that improves beef quality assurance is the ischiorectal fossa (IRF). The objective of this study was to determine whether administration of PGF2α in the IRF results in a similar physiologic response to administration of PGF2α given IM.
Yearling angus-cross heifers (n=112) were blocked by weight and randomly assigned within blocks to be injected with 5 mL PGF2α either IM in the neck or in the IRF. Blood samples were taken at 0, 8, 16, 24, 36, and 48 h post-injection. Serum samples were analyzed for progesterone concentration using a radioimmunoassay. Progesterone concentration curves for each heifer were plotted to determine luteolysis. The median times to luteolysis for neck and IRF injections were 18.1 hrs and 20.0 hrs, respectively (p=0.06).
Angus cross commercial beef cows (n=1471) at least 30 days post-partum were blocked by age and randomly assigned to be injected with 5 mL PGF2α either IM in the neck muscle or in IRF as part of a 7-Day CO-Synch + CIDR ovulation protocol. Pregnancy diagnosis was performed via ultrasound at 60 days post insemination. Results were analyzed with Proc Glimmix (SAS). Pregnancy rates for neck and IRF injections were 52.6% and 57.2%, respectively (p=0.06).
In summary, injection of PGF2α in the IRF for estrus synchronization and lysis of the corpus luteum did not differ from injection in the neck muscle. Utilizing the ischiorectal fossa as an injection site for PGF2α may be considered as an alternative that more closely aligns with beef quality assurance objectives. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/56575 |
| Date | 09 September 2015 |
| Creators | Holland, Sarah C. |
| Contributors | Veterinary Medicine, Swecker, William S. Jr., Whittier, William D., Clark-Deener, Sherrie |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis |
| Format | ETD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
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