In vitro assays were developed using small intestinal contents from broilers fed
maltodextrin in preparation for shipping. This was done to establish an effective
bactericidal dose of allicin or lysozyme as ingredients in maltodextrin-based feed. The
antimicrobials were added to overnight cultures of gut material bacteria from
maltodextrin fed broilers and a pure culture of Salmonella. Following this, lysozyme was
incorporated into maltodextrin feed at 0, 10 and 20 g.kg-1 of feed and offered for 9 h to 4
pens of 20 birds per treatment. Bacterial numbers were analyzed using Proc Mixed of
SAS. Allicin and lysozyme inhibited Enterobacteriaceae and Clostridium perfringens,
respectively, in vitro. Lysozyme showed the most promise; it reduced bacterial numbers
in nutrient broth. Feeding lysozyme-enriched maltodextrin for 9 h inhibited bacilli growth
(P<0.05) when evaluated using next generation sequencing. Lysozyme was effective in
reducing specific bacterial numbers in the gut of market-aged broilers / The project focused on ways to ensure poultry meat safety by controlling bacteria population in the gastrointestinal tract of market-aged broilers prior to shipping.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:NSHD.ca#10222/15131 |
| Date | 06 July 2012 |
| Creators | OFORI, REGINA |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Detected Language | English |
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