Farmers generally collect fresh plant materials from the wild for ethnoveterinary uses. They are
encouraged to harvest with caution and dry or cultivate important materials in order to protect the
biodiversity. These recommendations are not validated scientifically. The microplate method for
minimum inhibitory concentration (MIC) determination was used to compare wild with cultivated, and
fresh with dry plant materials. The MIC values obtained ranged from 1.25 to 0.01 mg/ml. MIC values ≤0.3
mg/ml were considered as cut off point between effective and none effective inhibition. The multilevel
linear models (hierarchical linear models), both unadjusted and adjusted models were employed. The
plant (name) was considered as level-2 or higher level, while the actual observation was level-1 or lower
level. The crude estimates of the odds ratio indicated that wild is significantly 0.57 times less likely than
garden to yield MIC values of more than 0.3 mg/ml (p-value = 0.005). Also, fresh are about 4.195 times
more likely than dry to yield MIC scores of more than 0.3 mg/ml (p-value < 0.001). Adjusting for
conditions “dry and fresh”, microbe and solvent; wild is significantly 0.52 times less likely than garden
to yield MIC values of more than 0.3 mg/ml (p-value = 0.003). On the other hand, when adjusting for “wild
or garden”, type of solvents and type of microbes; fresh is significantly 4.202 times more likely than dry
to yield MIC values of more than 0.3 mg/ml (p-value < 0.001). These results partially support farmers
claiming that wild plant materials are more potent than the grown ones. On the contrary, the results are
in favour of drying plant materials.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1000679 |
| Date | 05 November 2010 |
| Creators | Luseba, D, Letsoalo, ME, Katerere |
| Publisher | African Journal of Biotechnology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | |
| Rights | African Journal of Biotechnology |
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