This Master?s Thesis contains a description of the results of two research projects that were worked on over the course of two years. The first was an attempt to heterologously reconstitute the Bacillus subtilis RNase P RNA with the RNase P protein subunits of Methanothermobacter thermoautotrophicus, to identify a functional homology between one or more of the archaeal proteins with the Bacillus subtilis RNase P protein. Unfortunately, the reconstitution experiment could not be completed due to the poor quality of the pre-tRNAasp substrate synthesized. The second project dealt with comparative analysis of bacterial RNase P RNA. Eleven new RNase P RNA genes were amplified, sequenced, and their secondary structures derived by comparative analysis.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-09012003-153904 |
| Date | 01 October 2003 |
| Creators | Gantt, John Arthur |
| Contributors | Dr. Amy M. Grunden, Dr. Stephen J. Libby, Dr. James W. Brown |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-09012003-153904/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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