The process of transcription is quite complicated and involves many more steps than the classic textbook view of: Bind, melt, transcribe, release. We have studied the complex nature of transcription from the initiation of transcription through the transition from an unstable initiation complex to a stable, processive, elongation complex. We show that promoter release is not required to make the transition to a stable elongation complex. We show conclusively that a proposed model of transient excursion is not adequate for explaining the abortive cycling process that occurs in all known polymerases. We have proposed and tested a model that unites the processes of promoter release, collapse of the initially melted bubble, and proper RNA displacement. Additionally, we have challenged the existing model for the mechanism of inhibition by T7 lysozyme on T7 RNA polymerase.
| Identifer | oai:union.ndltd.org:UMASS/oai:scholarworks.umass.edu:dissertations-4149 |
| Date | 01 January 2006 |
| Creators | Esposito, Edward A |
| Publisher | ScholarWorks@UMass Amherst |
| Source Sets | University of Massachusetts, Amherst |
| Language | English |
| Detected Language | English |
| Type | text |
| Source | Doctoral Dissertations Available from Proquest |
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