Chromatin remodelers alter DNA-histone interactions in eukaryotic organisms, and have been well characterized in yeast and Arabidopsis. While there are maize proteins with similar domains as known remodelers, the ability of the maize proteins to alter nucleosome position has not been reported. Mutant alleles of genes encoding several maize proteins (RMR1, CHR101, CHR106, CHR127, CHR156, CHB102, and CHR120) with similar functional domains to known chromatin remodelers were identified. Altered expression of Chr101, Chr106, Chr127, Chr156, Chb102, and Chr120 was demonstrated in plants homozygous for the mutant alleles. These mutant genotypes were subjected to nucleosome position analysis to determine if misregulation of putative maize chromatin proteins would lead to altered DNA-histone interactions. Nucleosome position changes were observed in plants homozygous for chr101, chr106, chr127, chr156, chb102, and chr120 mutant alleles, suggesting that CHR101, CHR106, CHR127, CHR156, CHB102, and CHR120 may affect chromatin structure. The role of RNA polymerases in altering DNA-histone interactions was also tested. Changes in nucleosome position were demonstrated in homozygous mop2-1 individuals. These changes were demonstrated at the b1 tandem repeats and at newly identified loci. While the α-amanitin-inhibited RNA polymerase II demonstrated reduced expression of an RNA polymerase II transcribed gene, no changes in nucleosome position were detected in the α-amanitin-treated plants. Additionally, differential DNA-histone interactions and altered expression of putative chromatin remodelers in different maize haplotypes suggest a role for differentially expressed chromatin proteins in haplotype-specific variation. / A Dissertation submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Doctor of Philosophy. / Summer Semester 2017. / July 14, 2017. / chromatin, chromatin remodelers, nucleosome position, transcription start site, Zea mays / Includes bibliographical references. / Karen M. McGinnis, Professor Directing Dissertation; Myra M. Hurt, University Representative; Hank W. Bass, Committee Member; Brian P. Chadwick, Committee Member; Jonathan H. Dennis, Committee Member.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_552339 |
| Contributors | Stroud, Linda Kozma (authoraut), McGinnis, Karen M. (professor directing dissertation), Hurt, Myra M. (university representative), Bass, Hank W. (committee member), Chadwick, Brian P. (committee member), Dennis, Jonathan Hancock (committee member), Florida State University (degree granting institution), College of Arts and Sciences (degree granting college), Department of Biological Science (degree granting departmentdgg) |
| Publisher | Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text, doctoral thesis |
| Format | 1 online resource (112 pages), computer, application/pdf |
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