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Optimalizace izolace plazmidové DNA pomocí magnetických částic / Optimization of plasmid DNA isolation by magnetic particles

The theoretical part summarizes information on the isolation and purification of plasmid DNA and nucleic acids as. Plasmid DNA is often used in gene engineering as a vector for the transfer of a particular gene. Its insulation and transportation in sufficient quality is crucial for other processes associated with it. Isolation and survival of pDNA using magnetic carriers of different concentrations of PEG 8000 in combination with 1M NaCl was investigated in experimental parts. Furthermore, the isolation of pDNA using commercial kits was examined.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:401929
Date January 2019
CreatorsChlopková, Barbora
ContributorsZemanová, Jana, Brázda, Václav
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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