A water-methanol solution of glycine and scD-glucose was refluxed for seven hr. The solvent was evaporated under vacuum at room temperature and the residue was dialyzed against distilled water. After dialysis, the solvent was evaporated under vacuum at room temperature. The non-dialyzable fraction was further fractionated by gel filtration. The process yielded three polymeric materials (10,000 $<$ M $<$ 20,000) whose purity was verified by HPLC. The isolated polymers were further analyzed by UV-VIS and FTIR spectroscopy and by pyrolysis/GC/MS. Elemental analysis indicated that polymer A has the following empirical formula $ rm C sb6H sb{11}N sb1O sb4$ and polymers B1 and B2 have the same empirical formula as glucose $ rm C sb1H sb2O sb1.$ The origin of nitrogen containing polymer A was assigned to Amadori intermediate or to some of its derivatives and the origin of polymers B1 and B2 was assigned to Glucosone and to 3- or 1-deoxyglucosones; common non-nitrogen containing reactive intermediates during Maillard reaction. Plausible mechanisms were proposed for the formation of polymers.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27535 |
| Date | January 1997 |
| Creators | Kaminski, Eva. |
| Contributors | Yaylayan, V. A. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Food Science and Agricultural Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001615786, proquestno: MQ37134, Theses scanned by UMI/ProQuest. |
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