High level of agricultural products processing and especially better storage of products currently represent one of the hottest topics. At the same time, there is a lot of effort to grow crops without chemical substances. Root vegetables, which are consumed a lot, are prone to many harmful and damaging influences. There are several risk factors, including a long vegetation period. This creates an opportunity to use natural substances, in particular essential oils. Their effects are used in various different fields because they are safe for the environment as well as for the human health and the area of food commodities treatment. This study focuses on antibacterial activity testing of several essential oils: satureja, cinnamon, clove, thyme and oregano. Carrot, garden parsley and celery were chosen as representatives of root vegetables. The antibacterial activity was measured by the broth microdilution method. Even though the vegetables were inoculated with pathogenic bacteria, putrefaction did not develop in the specific places. Therefore an isolate from the parsley and the celery was then used to identify several other microorganisms by the MALDI TOF mass spectrometry. Consequently, the minimum inhibitory concentration of essential oils was again tested against these bacteria in in vitro conditions.
There was a demonstrable positive result: the most frequent minimum inhibitory concentration of the cinnamon essential oil was 0.128 mg/ml. At this level the essential oil inhibited eight out of the seventeen tested microorganisms. The other tested essential oils showed some inhibition activity at least against one bacterium in in vitro conditions.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:256802 |
| Date | January 2017 |
| Creators | Krondlová, Marie |
| Contributors | Klouček, Pavel, Jakub, Jakub |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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