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Previous issue date: 2012-06-11 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study evaluates the biosurfactants production from cassava wastewater, an agro industrial residue, to be used as carbon source. Using a factorial design 24-1 (half fraction), 10 tests were performed using Pseudomonas aeruginosa AP029/GVII-A in submerged batch cultivation in rotating incubator (shaker). The influence of factors (temperature, agitation, aeration ratio and concentration of cultivation medium) at two different levels for the synthesis of the biosurfactant. Samples were collected throughout the cultivation by 132 hours of fermentation were completed. The best outcome was intended by following production through substrate consumption, dry matter, reduction of surface tension (ring method) and emulsification index. The kinetics of microorganism was assessed for the carbon source used. The results showed that the cassava wastewater is a well assimilable substrate for the production of biotensoactive, reaching 91 % of consumption by the micro-organism under study. The growth temperature was found to be one of the leading factors in the synthesis of the metabolite, followed by aeration and also due to the agitation. The best results showed a 30 % reduction in surface tension (% RTS) for the environment, reaching values of 30 mN/m; 3.0 g /L of biomass and emulsifying index greater than 65 %. The metabolite synthesized still remained stable for different salt concentrations (1, 5 and 10 % w/ v) and alkaline pH (8-10). / O presente trabalho avalia a produ??o de biossurfactantes a partir de manipueira, um res?duo agroindustrial a ser utilizado como fonte de carbono. Empregando um planejamento fatorial 24-1 (fra??o meia), 10 ensaios foram realizados utilizando Pseudomonas aeruginosa AP029-GVIIA em cultivo descont?nuo submerso em incubador rotat?rio (shaker). Foi analisada a influ?ncia dos fatores (temperatura, agita??o, raz?o de aera??o e concentra??o do substrato) em dois diferentes n?veis para a s?ntese do biossurfactante. Amostras foram coletadas em intervalos regulares durante o cultivo at? 132 horas de fermenta??o. Buscou-se o melhor resultado acompanhando a produ??o atrav?s do consumo de substrato, massa seca, redu??o da tens?o superficial (m?todo do anel) e ?ndice de emulsifica??o. O comportamento cin?tico do micro-organismo foi avaliado para a fonte de carbono utilizada. Os resultados mostraram que a manipueira ? bastante assimil?vel e potencial substrato na produ??o do biotensoativo, onde se observou 91% de consumo pelo micro-organismo em estudo. A temperatura de cultivo revelou ser um dos fatores preponderantes na s?ntese do metab?lito, acompanhada pela raz?o de aera??o e agita??o. Os melhores resultados mostraram redu??o de 30% da tens?o superficial (%RTS) em rela??o ao meio inicial, alcan?ando valores de 31 mN/m; 3,0 g/L de biomassa e ?ndice de emulsifica??o superior a 65%. O metab?lito sintetizado ainda se mostrou est?vel para diferentes concentra??es salinas (1, 5 e 10% m/v) e pH alcalino (8-10).
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/15917 |
| Date | 11 June 2012 |
| Creators | Bezerra, M?rcio Silva |
| Contributors | CPF:87510383404, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799564Y2, Correia, Roberta Targino Pinto, CPF:84702885472, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798196E6, Assis, Cristiane Fernandes de, CPF:03143301600, http://lattes.cnpq.br/0034694007210837, Lima, Anita Maria de, CPF:59763981468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767606U6, Silva, Fl?vio Luiz Honorato da, CPF:42579325468, http://lattes.cnpq.br/2082780006180637, Macedo, Gorete Ribeiro de, Santos, Everaldo Silvino dos |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Engenharia Qu?mica, UFRN, BR, Pesquisa e Desenvolvimento de Tecnologias Regionais |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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