The present study explored the effect of three anti-tumor drugs (cisplatin, fluorouracil, and temozolomide) on viability and cytosolic free Ca2+ concentrations ([Ca2+]i) in OC2 human oral cancer cells. The effect of cisplatin related mitogen-activated protein kinases (MAPKs) phosphorylation was also examined. Cisplatin at concentration of 25-150 £gM decreased viability in a concentration-dependent manner, and so did fluorouracil (50-1000 £gM) and temozolomide (50-600 £gM). The three anti-tumor drugs all failed to induce a [Ca2+]i increase; thus it seemed that these drugs induced cell death via Ca2+-independent pathways. Immunoblotting showed that OC2 cells have background phospho-ERK, phospho-JNK and phospho-p38 MAPKs. It was found that cisplatin influenced the phosphorylation of ERK, JNK and p38 MAPKs at different time points.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0903108-013633 |
| Date | 03 September 2008 |
| Creators | Su, Hsing-Hao |
| Contributors | Chung-Ren Jan, Chung-Lung Cho, Ching-Mei Hsu, Jiin-Tsuey Cheng, Ching-Mei Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0903108-013633 |
| Rights | withheld, Copyright information available at source archive |
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