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Construction of a Physical Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238

In order to gain a better understanding of this microorganismand its role in human pathogenesis, a physical map of Moraxella catarrhalis type strain ATCC25238 was constructed using pulsed field gel electrophoresis (PFGE) in combination with Southern hybridization techniques. Restriction endonucleases Not I, Rsr II, and Sma I were used to digest the chromosomal DNA. An overlapping circular map was generated by cross-hybridization of isolated radiolabeled fragments of Moraxella catarrhalis genomic DNA to dried PFGE gels. The number and location of the 16S and 23S ribosomal RNA genes were determined by digestion with l-Ceul enzyme and by Southern hybridization.
Virulence-associated genes, the gene for β-lactamase, and housekeeping genes were also placed onto the physical map.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc279104
Date05 1900
CreatorsNguyen, Kim Thuy
ContributorsFarinha, Mark A., O'Donovan, Gerard A., Goven, Arthur James, 1950-
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvi, 49 leaves : ill., Text
RightsPublic, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Nguyen, Kim Thuy

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