Pineacea is the largest family of conifers that includes 51 species of Abies
which is the second largest genus after Pinus.
There are six native taxa in Turkey belonging to this genus. Four of these
taxa (Abies cilicica subsp. isaurica, Abies nordmanniana subsp.
bornmü / lleriana, Abies nordmanniana subsp. equi-trojani, Abies x olcayana)
are endemic and considered as low risk (LR) species according to the IUCN
criteria.
To determine the phylogenetic relationship in Abies spp. in Turkey, 18
populations of different taxa were collected from their natural distribution
areas in Turkey. The matK gene regions of chloroplast DNA (cpDNA) were
studied comparatively to reveal the genetic relationship among Turkish fir
species. The available sequences from the NCBI database for the matK
region of the other Abies species in the world were also investigated
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comparatively with the sequences from Turkish firs. With the matK sequence
data, a phylogenetic tree was constructed for the fir species and the
molecular diversity parameters such as conserved and variable sites,
nucleotide diversity, and evolutionary divergence were estimated using the
MEGA software.
The results indicated that there are no variable sites among Turkish firs with
regard to matK regions of cpDNA. It appears that the matK region of cpDNA
for Turkish firs is highly conserved.
Since sequence data for all matK regions were not available from the NCBI
data base, the phylogentic analysis with the sequence data of matK were
comparatively analyzed in all firs including Turkish firs. According to matK1
region, the results showed that there were three major clades. One of the
clades included all Turkish fir taxa and one species from European firs,
A.numidica / however, A.holopylla, A.firma, A.veitchii, A.sachalinensis,
A.nephrolepis, A.lasiocarpa, A.koreana, A.homolephis, A.fraseri, A.fargesii,
A.sibirica and A.fabri formed in another clade. In addition to this, A.mariesii,
A.hidalgensis, A.bracteata, A.alba formed in different major clade. According
to matK1 region the results showed that Turkish firs and European firs are
closer to each other. Furthermore, based on matK2 region, the results
indicated that Turkish firs formed a monophyletic group.The other fir species,
with respect to matK2 regions, formed different clades from Turkish firs.
The results based on matK region suggest that all Turkish firs may have
evolved from single ancestral fir species and the matK gene region appears
to be highly conserved region in fir species.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12613642/index.pdf |
| Date | 01 September 2011 |
| Creators | Ates, Mevlude Alev |
| Contributors | Kaya, Zeki |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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