Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008. / Cataloged from PDF version of thesis. / Includes bibliographical references (p. 27-28). / In this study biomaterial scaffolds for regeneration of nucleus pulposus were developed by freeze drying slurries with different proportions of collagen II (CII), chondroitin-6-sulfate (CS), and hyaluronic acid (HA). The scaffolds were analyzed using biochemical assays to determine final composition. Chemically cross-linked scaffolds were analyzed to determine pore size and cross-link density. It was determined that every material type contained large enough pore size (275 gm) to seed nucleus pulposus cells and mesenchymal stem cells. The addition of CS to the scaffold increased pore size. It was also found that increasing levels of CS and HA resulted in lower cross-link density. These materials will be used next in In Vitro studies to determine their viability as regenerative tissue engineering constructs. / by Jacob A. Simson. / S.B.
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/57874 |
| Date | January 2008 |
| Creators | Simson, Jacob A |
| Contributors | Myron Spector., Massachusetts Institute of Technology. Dept. of Materials Science and Engineering., Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 28 p., application/pdf |
| Rights | M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission., http://dspace.mit.edu/handle/1721.1/7582 |
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