Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, June 2005. / Includes bibliographical references (p. 38-39). / In this thesis, the chemical/mechanical properties and biocompatibility of gelatin were investigated to produce a gelatin scaffold for the release of bone morphogenetic proteins (BMPs) from composite particles. This delivery system, designed to regenerate bone, holds much promise as an alternative to bone grafts. The chemical properties of gelatin were examined through zeta potential measurements, swelling studies, optical microscopy, environmental scanning electron microscopy (ESEM), and collagenase degradation. Compressive tests and mercury porosimetry were performed to study the mechanical and structural properties of the scaffold. The biocompatibility of the scaffold was determined through cell optical imaging and DNA quantification studies. Based on findings of this research, the material choices were made and the synthesis method for the gelatin scaffold was developed. Gelatin A, 300B, derived from bovine collagen, with an isoelectric point of [approx.] 9, was selected. Crosslinking was accomplished by reacting 10 w/v% glutaraldehyde with 10 w/v% gelatin solution. The most effective crosslinking condition was found to be 5 hours at room temperature. Glycine rinses were conducted to cap any non- reacted (toxic) aldehyde groups, and the necessary length of time was found to be at least 48 hours at 37⁰C. Finally, based on pore size distribution and mechanical stability, an optimal lyophilization method was developed with initial freezing at -20⁰C for 1 day, followed by lyophilization of the scaffold for 1-2 days. In terms of mechanical properties of the gelatin and amount of protein delivered, the most effective loading of poly(lactic-co-glycolic acid)/apatite/protein composite particles was found to be 10% of the mass of the gelatin. / by Elizabeth A. Hager. / S.B.
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/32844 |
| Date | January 2005 |
| Creators | Hager, Elizabeth A. (Elizabeth Ann) |
| Contributors | Jackie Y. Ying., Massachusetts Institute of Technology. Dept. of Materials Science and Engineering., Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 39 p., 1780617 bytes, 1780204 bytes, application/pdf, application/pdf, application/pdf |
| Rights | M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission., http://dspace.mit.edu/handle/1721.1/7582 |
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