Mestrado em Controlo de Qualidade / MSc in Quality Control / Aiming for new compounds with antitumor activity, the synthesis of prenylated xanthones and prenylated
and geranylated flavones was recently achieved on CEQOFFUP. In this work the potential antitumor
activity of these compounds in three tumor cell lines, namely MCF-7 (human breast cancer cells
expressing estrogen receptors (ER+)), MDA-MB-231 (human breast cancer cells non expressing estrogen
receptors (ER-)) and NCI-H460 (non-small cell lung cancer) was evaluated. Structure-activity relationships
were established highlighting the influence of prenylation and geranylation.
Concerning xanthones, prenylation of 3,4-dihydroxyxanthone (XXIX) furnished more potent and selective
derivates for MCF-7 (ER+) cells than the original oxygenated xanthone. Xanthone derivate XP13 showed
the strongest inhibitory effect on the growth of breast adenocarcinoma cell line ER+, MCF-7 (GI50 = 5,3
M). Thus, potential estrogenic/antiestrogenic properties were investigated for this compound. No
proliferative effect at low concentrations was observed for XP13 in experiments performed in steroid-free
medium (RPMI-SFM). However, when high concentrations of XP13 were used, this prenylated xanthone
inhibit cancer cell growth in a dose-dependent manner, being more active on MCF-7 (ER+) cell line than
on MDA-MB-231 (ER -) cell line. This antiproliferative effect was not influenced by the culture medium
(steroid (RPMI) or steroid-free medium (RPMI-SFM)). From these results it can be inferred that XP13 does
not directly act on the estrogen receptor, suggesting that it could interfere with other signaling pathways.
Moreover, XP13 enhanced the growth inhibitory action of 4-hydroxytamoxifen (4-OHT, XIV), a partial
antiestrogen in estrogen sensitive breast cancer cells.
Concerning flavone derivates, none of the six flavones investigated, that were resulted from prenylation
and geranylation of baicalein (BAIC, XIX), presented a higher cytotoxic effect on all tumor cellular lines
(MCF-7 (ER+), MDA-MB-231 (ER -) and NCI-H460) when compared to the original oxygenated flavone
BAIC (XIX). However, monoprenylation in C(7) conduced to a flavone (FP2) with a selective inhibitory
effect on the growth of MCF-7 (ER+) cells. Possible estrogenic/antiestrogenic properties were investigated
for this compound. It was verified that in steroid-free medium (RPMI-SFM) experiments, FP2 presented a
biphasic effect in vitro growth on the ER-positive MCF-7 cell line. Although at low concentrations this
flavone has stimulated cell growth, at high concentrations a cell growth inhibition was observed. Then, the
effect of FP2 in combination with E2 was examinated. Results showed that FP2 suppressed at low
concentrations, the mitogenic effect enhanced by estrogenic stimulation, suggesting a competition for
ERs. Also, the FP2 cancer cell growth inhibitory effect on MCF-7 (ER+) cells was stronger when assed in
steroid free medium, i.e., in the absence of estrogenic stimulation. These results suggest a direct
involvement of estrogen receptor in the proliferative/antiproliferative effect of flavone FP2. Moreover, FP2
enhanced the growth inhibitory action of partial (4-OHT, XIV) and pure (ICI 182,780, XII) antiestrogens in
estrogen sensitive breast cancer cells.
These results were consistent with previous reports of prenylated flavones and disclose for prenylated
xanthones effects compatible with antiestrogenic activity. Thus, the present work represents a promising
contribution for the prevention and treatment of hormone-dependent breast cancer.
| Identifer | oai:union.ndltd.org:up.pt/oai:repositorio-aberto.up.pt:10216/10802 |
| Date | 09 January 2008 |
| Creators | Camões, Ana Catarina Dias Gonçalves Sobral |
| Publisher | Faculdade de Farmácia da Universidade do Porto, FFUP |
| Source Sets | Universidade do Porto |
| Language | Portuguese |
| Detected Language | English |
| Type | Dissertação |
| Format | application/pdf, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf |
| Rights | openAccess |
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