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Previous issue date: 2016-12-13 / Oral candidiasis is a fungal infection caused by yeasts of the genus Candida, with
C. albicans being the main pathogen. The emergence of resistant species against
the available therapeutic arsenal as well as its adverse effects has driven new
research on natural products. In view of this problem, the present study sought to
investigate the antifungal activity of essential oil (OE) obtained from the leaves of
Melissa officinalis L (lemon balm), isolated and associated with antifungal agents
licensed on C. albicans strains. Two strains of the American Type Culture
Collection (ATCC) and eight from the oral cavity (LM) were selected. The
microdilution and sowing of subcultures were used to determine, respectively, the
Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration
(CFM). The antifungigram was performed using the disc diffusion technique with
the antifungal agents nystatin, amphotericin B, miconazole and fluconazole,
evaluating them in isolation and associated with OE. MIC and MFC ranged from
64 to 128 μg/mL and 256 to 512 μg/mL, respectively. The presence of resistance
of the strains to the antifungal agents nistatin (LM-38 and LM-65), amphotericin B
(ATCC-76485, LM-3A, LM-38, LM-62 and LM -65) and fluconazole (LM-1A, LM-
1B, LM-38, LM-62 and LM-65). In the association tests, the results showed the
influence of the essential oil on the antifungal activity, with synergism (S),
indifference (I) and antagonism (A) being observed. The percentages found for
each antifungal in the order (S, I and A) were: nystatin (30%, 30% , 40%),
amphotericin B (30%, 50%, 20%), fluconazole (20% ,40% , 40%) and miconazole
(100%, 0,0). In view of the above, it is concluded that the OO of M. Officinalis may
become a promising product in the fight against oral candidiasis, since it has a
strong antifungal activity against Candida albicans and that the use of this product
associated with the licensed antifungal can, in some situations, potentiate its
effectiveness of use in treatment. / A candidíase oral é uma infecção fungica causada por leveduras do gênero
Candida, sendo a espécie C. albicans apontada como principal patógeno. O
surgimento de espécies resistentes frente ao arsenal terapêutico disponível assim
como seus efeitos adversos tem impulsionado novas pesquisas voltadas para os
produtos naturais. Diante dessa problemática o presente trabalho buscou
investigar a atividade antifúngica do óleo essencial (OE) obtido das folhas de
Melissa officinalis L (erva-cidreira), isolado e associado a antifúngicos licenciados
sobre cepas de C. albicans. Para tanto, foram selecionadas duas cepas da
American Type Culture Collection (ATCC) e oito provenientes de cavidade oral
(LM). Utilizou-se a técnica da microdiluição e semeadura de subcultivos para
determinar, respectivamente, a Concentração Inibitória Mínima (CIM) e a
Concentração Fungicida Mínima (CFM). O antifungigrama foi realizado a partir da
técnica de difusão de disco com os antifúngicos nistatina, anfotericina B,
miconazol e fluconazol avaliando-os de forma isolada e associada ao OE. A CIM
e CFM variaram, respectivamente, de 64 a 128 μg/mL e 256 a 512 μg/mL. Quanto
ao teste de sensibilidade de forma isolada observou-se a presença de resistência
das cepas aos antifúngicos nistatina (LM-38 e LM-65), anfotericina B (ATCC-
76485, LM-3A, LM-38, LM-62 e LM-65) e fluconazol (LM-1A, LM-1B, LM-38, LM-
62 e LM-65). Nos ensaios de associação os resultados mostraram influência do
óleo essencial sobre a atividade dos antifúngicos sendo observado sinergismo
(S), indiferença (I) e antagonismo (A). As porcentagens encontradas para cada
antifúngico, na ordem (S, I e A), foram: nistatina (30%, 30% e 40%), anfotericina B
(30%, 50%, 20%), fluconazol (20%, 40% e 40%) e miconazol (100%,0,0). Perante
o exposto conclui-se que o OE de M. Officinalis pode se tornar um produto
promissor no combate a candidose oral, visto que apresenta forte atividade
antifungica contra Candida albicans e que o uso desse produto associado à
antifungicos licenciados pode, em algumas situações, potencializar sua
efetividade de uso no tratamento.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede.biblioteca.ufpb.br:tede/8855 |
| Date | 13 December 2016 |
| Creators | Leitão, Maíra Catherine de Negreiros |
| Contributors | Lima, Edeltrudes de Oliveira |
| Publisher | Universidade Federal da Paraíba, Programa de Pós-Graduação em Odontologia, UFPB, Brasil, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFPB, instname:Universidade Federal da Paraíba, instacron:UFPB |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7411869720500764667, 600, 600, 600, 1726686701291727373, -2070498469879244349 |
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