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Treatment of nonspecific DNA-protein contacts and application to the excision mechanism of a unique human DNA glycosylase

This thesis concentrates on understanding how individual nonspecific DNA–protein
contacts are used in the excision mechanism of the human DNA repair enzyme, alkyladenine
DNA glycosylase (AAG). Initially, studies focus on understanding the structure and
magnitude of these fundamentally different DNA–protein stacking and T-shaped
interactions to be applied to the active site of AAG. High-level ab initio techniques revealed
fundamental knowledge about the structure and magnitude of these distinctly different –
and +– contacts between (one or two) conjugated amino acid(s) and one nucleobase.
Additionally, the mechanism used by AAG to excise (neutral and cationic) damaged
nucleotides was investigated using a hybrid ONIOM approach. Reaction potential energy
surfaces reveal that AAG prefers to excise both neutral and cationic substrates through a
concerted mechanism, yet the nonspecific contacts present in the active site are only
catalytic for the cleavage of the neutral substrates. / xvi, 195 leaves : ill. (some col.) ; 29 cm + 1 CD-ROM

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:ALU.w.uleth.ca/dspace#10133/2636
Date January 2011
CreatorsRutledge, Lesley R, University of Lethbridge. Faculty of Arts and Science
ContributorsWetmore, Stacey
PublisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2011, Arts and Science, Department of Chemistry and Biochemistry
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Languageen_US
Detected LanguageEnglish
TypeThesis
RelationThesis (University of Lethbridge. Faculty of Arts and Science)

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