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Previous issue date: 2016-05-04 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The family Cactaceae has approximately 100 genera and 1500 species predominantly American and distributed by a wide variety of climates and ecosystems. Due to the high singularity in terms of genres and endemic species as well as the potential use of these plants for various purposes, studies on the in vitro propagation and ex situ conservation of these species must be established. Thus, the aim of this study was to understand the anatomical, physiological and molecular changes associated with the propagation and conservation in vitro Discocactus zehntneri subsp boomianus and Stephanocereus leutezlburgii, species endemic cactus of Bahia with ornamental potential. The regulators applied not exceeded the control in the induction of shoots/explant in both species and for S. luetzelburgii combination of cytokinins and auxins hiperidricos increased the percentage of shoots that did not survive after acclimatization. Normal shoots of this species have 100% survival in ex vitro conditions. The callus formation was enhanced by adding auxin to the nutrient medium for the two species analyzed and calluses with potential for in vitro morphogenesis were obtained. The proteomic analysis revealed the marker proteins differential expression of somatic embryogenesis in two strains of S. luetetzelburgii calluses. The protocol for cryopreservation D. zehntneri shoots was not effective, however the seed storage either in liquid nitrogen conditions as for ultrafreezer maintained physiological quality after storage for 360 and 180 days, respectively. Osmotic agents were effective in inhibiting in vitro growth of the species for a year without loss of viability. / A fam?lia Cactaceae apresenta cerca de 100 g?neros e 1500 esp?cies predominantemente americanas e distribu?das por uma ampla diversidade de climas e ecossistemas. Devido ? alta singularidade em termos de g?neros e esp?cies end?micas bem como o uso potencial destas plantas para diversas finalidades, estudos acerca da propaga??o in vitro e conserva??o ex situ dessas esp?cies devem ser estabelecidos. Desta forma, o objetivo geral deste trabalho foi compreender as mudan?as anat?micas, fisiol?gicas e moleculares associadas ? propaga??o e conserva??o in vitro de Discocactus zehntneri subsp boomianus e Stephanocereus leutezlburgii, esp?cies de cact?ceas end?micas da Bahia com potencial ornamental. Os reguladores aplicados n?o superaram o controle na indu??o de brotos/explante em ambas as esp?cies e para S. luetzelburgii a combina??o de citocininas e auxinas aumentaram o percentual de brotos hiperidricos que n?o sobreviveram ap?s a aclimatiza??o. Brotos normais dessa esp?cie apresentaram 100% de sobreviv?ncia em condi??es ex vitro. A calog?nese foi potencializada pela adi??o de auxinas ao meio nutritivo para as duas esp?cies analisadas e calos com potencial para morfog?nese in vitro foram obtidos. A an?lise prote?mica revelou a express?o diferencial de prote?nas marcadoras da embriog?nese som?tica em duas linhagens de calos de S. luetetzelburgii. O protocolo para criopreserva??o de brotos de D. zehntneri n?o foi eficiente, no entanto a armazenamento de sementes tanto em condi??es de nitrog?nio l?quido quanto em ultrafreezer mantiveram a qualidade fisiol?gica ap?s o armazenamento por 360 e 180 dias, respectivamente. Os agentes osm?ticos foram eficientes em inibir o crescimento in vitro da esp?cie por um ano sem perda na viabilidade.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/360 |
| Date | 04 May 2016 |
| Creators | Marchi, Maria Nazar? Guimar?es |
| Contributors | Santana, Jos? Raniere Ferreira de |
| Publisher | Universidade Estadual de Feira de Santana, Doutorado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -3267524094848184949, 600, 600, 600, 600, 5026123383450589282, -1634559385931244697, 3590462550136975366 |
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