A gene duplication occurs in Salmonella typhimurium which involves about 30% of the genome. The frequency of formation and loss of this duplication was determined in a Rec.A⁻ strain and a PolA⁻ strain and compared to that of a RecA⁺, PolA⁺ strain. The frequency of formation was found to be reduced in both RecA⁻ and PolA⁻. strains. Loss of the duplication was eliminated in the RecA⁻ strain, while the frequency of loss was found to be greater in a PolA⁻ strain than in the PolA⁺ strain.
The effect of UV irradiation on the formation of duplication was also compared for the three strains. There is an increase in the frequency of formation in both the PolA⁺, RecA⁺ and PolA⁻, RecA⁺ strains, the increase being greater in the PolA⁻ strain. The frequency of formation is reduced in the RecA⁻ strain. It is postulated that recA-dependent repair pathways are involved in the formation of the duplication, but there is at least one other pathway available. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/70470 |
| Date | January 1979 |
| Creators | Winfield, Suzanne L. |
| Contributors | Microbiology |
| Publisher | Virginia Polytechnic Institute and State University |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | en_US |
| Detected Language | English |
| Type | Thesis, Text |
| Format | iv, 58, [1] leaves, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 5526141 |
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