The plant cell wall consists of several layers: cellulose, hemicellulose, lignin and pectin. These biopolymers are degraded by many microorganisms. Extracellular enzymes are used for biodegradation by microorganisms. This thesis was focused on studying the impact of cultivation conditions on the production of extracellular enzymes at carboxymethyl cellulase and pectin when a mixed thermophilic culture containing Bacillus and Thermus microorganisms is used. The cultivation was carried out in flasks on a shaking machine with a shaking speed of 99 min-1 at a temperature of 60°C. The monitoring covered cellulolytic and polygalacturonase activities, protein concentration by the Biuret method, concentration of reducing substances by the Somogyi and Nelson methods, and the temperature optimum.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216414 |
| Date | January 2008 |
| Creators | Klašková, Lenka |
| Contributors | Zemanová, Jana, Babák, Libor |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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