The morphologic variability seen in the domestic dog, Canis lupus familiaris, is
unique among mammals. Selective pressures imposed by humans have divided dogs into
almost 400 separate breeds. Selection has also led to the development of approximately
450 hereditary diseases, many of which are limited to specific breeds. Over half of these
diseases present with similar clinical characteristics to those of many human hereditary
diseases, making the dog an ideal model for study of the genetic bases of such diseases.
Many diseases do not have candidate genes or have too many candidates to characterize.
This is exacerbated in complex diseases that are caused by several genes. Whole-genome
scans can provide insight into diseases by identifying marker(s) that co-segregate with a
disease phenotype. The Minimal Screening Set - 2 (MSS-2) is the most recent set of
microsatellites suitable for whole-genome screens. The first objective of this work was
to streamline genomic screens in order to efficiently analyze large numbers of animals.
To this end, chromosome-specific microsatellite panels were developed for the MSS-2.
Canine hip dysplasia (CHD) is the most common orthopedic disease of the dog.
CHD primarily affects medium and large breed dogs, but is found in almost every breed.
The major objective of this work was to use linkage analysis to identify chromosomal
regions that contain genes that are involved in CHD. Two populations were screened
using the MSS-2. The first was a small family of Boykin Spaniels, though no markers were statistically significant in a whole-genome screen. An outcrossed pedigree of
Greyhound/Labrador Retrievers was created for quantitative trait loci (QTL) mapping of
CHD. The informativeness of markers in the F2 and backcrossed generations were
calculated to show the utility of using such a population. Other factors that affect the
power of this pedigree to identify QTL were also highlighted. Chromosomes that were
identified in a previous screen as harboring putative QTLs were examined using the
chromosome-specific panels to further define and confirm the regions of interest.
Although no markers reached statistical significance, several areas of interest were
identified.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/4878 |
| Date | 25 April 2007 |
| Creators | Tsai, Kate Leanne |
| Contributors | Murphy, Keith E. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | 1273045 bytes, electronic, application/pdf, born digital |
Page generated in 0.0018 seconds