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Previous issue date: 2016-03-14 / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Musca domestica é um díptero que apresenta sérios riscos à saúde pública e ambiental. Essa espécie transmite diversos patógenos, é causadora de miíases em humanos e animais além de causar prejuízos econômicos. O uso de inseticidas químicos causa danos ambientais e possui eficácia questionável em M. domestica. Inseticidas biológicos de origem bacteriana apresentam uma boa alternativa aos inseticidas químicos em relação aos impactos ambientais, as espécies Brevibacillus laterosporus, Bacillus thuringiensis e Lysinibacillus sphaericus demonstram ação inseticida em diversas ordens de insetos e podem apresentar atividade inseticida efetiva em M. domestica. Nove estirpes de bactérias foram testadas em M. domestica e destas três estirpes: BL102, BTI193A e BTK176 apresentaram valores de mortalidade maiores que 50% e por isso tiveram os efeitos subletais investigados. Em relação a massa das pupas, nenhuma das estirpes apresentou variação significativa na média da massa pupal, quando comparadas às médias dos controles puro e água
O período de desenvolvimento também não se diferenciou de forma estatisticamente relevante dos dois controles em nenhum tratamento. As três estirpes testadas apresentaram mortalidade estatisticamente superior às mortalidades dos dois controles com LC50 nas concentrações de 12.40x108 UFC/mL para BTI193, 4.76x108 UFC/mL para BTK176 e 4.16x108 UFC/mL para BL102. As micrografias de transmissão demonstraram que todas as estirpes apresentam o perfil de danos celulares já descritos para essas espécies de bactérias entomopatogênicas, como elevada vacuolização do citoplasma, além de desorganização das organelas celulares, porém somente as estirpes de B. thuringiensis apresentaram deformação e interrupção das microvilosidades e conteúdo citoplasmático extravasado para o lúmen intestinal. Os resultados obtidos com de B. laterosporus e B. thuringiensis sugerem que essas estirpes são um agentes de controle biológico promissores para M. domestica
Abstract: Musca domestica is a dipteran who presentes a serious risk to public and environmental health. This species carries diseases, causes myiasis in humans and animals and may cause economic losses. The use of chemical insecticides, beyond causing environmental damage, have questionable efficacy in M. domestica. Biologic insecticides of bacterial origin presents a good choice over chemical insecticides regarding environmental impact. Brevibacillus laterosporus, Bacillus thuringiensis and Lysinibacillus sphaericus species show insecticide action in several insect orders and may presente insecticide effectiveness in M. domestica. Nine strains of bacteria were tested for mortality where three strains: BL102, BTI193 and BTK176 showed values above 50%. These strains were submitted to sub lethal effects experiments. In relation to pupal weight, none of the strains showed statistical variation when compared to pure and water controls
Development time was statistically undifferentiated from both controls in every treatment tested. All the tested strains showed statistically higher mortality when compared to both controls, showing LC50 concentrations of 12.40x108 CFU/mL for BTI193, 4.76x108 CFU/mL for BTK176 and 4.16x108 CFU/mL for BL102. Transmission micrographs showed that every strain presented cell damage profile established for these species of entomopathogenic bacteria, such as the high level of cytoplasm vacuolization and disorganization of cell organelles, however, only B. thuringiensis strains showed microvilli deformation and disruption and cytoplasmic content extruded into intestinal lumen. All these observations suggest that tested B. laterosporus and B. thuringiensis strains are promising agents to the biological control of M. domestica / Musca domestica
is a dipteran who presentes a serious
risk
to public and environmental
health.
This species carries diseases,
causes myiasis in humans and animals and may
cause
economic
losses.
The use of chemical insecticides,
beyond
causing environmental
damage, have questionable
efficacy in
M. domestica
.
Biologic insecticides of bacterial
origin presents a good choice over chemical insecticides regarding environmental
impact
.
Brevibacillus laterosporus
,
Bacillus thuringiensis
and
Ly
sinibacillus sphaericus
species show insecticide action in several insect orders and may presente insecticide
effectiveness in
M. domestica
.
Nine
strains of bacteria were tested
for mortality
where
three strains: BL102, BTI193
and
BTK176
showed values
above 50%
.
These strains
were submitted to
sub lethal effects
experiments
.
In relation to pupal weight
,
none of
the strains showed statis
ti
cal
variation when compared to pure
and water
controls.
Development time was statis
ti
cally undifferentiated from bot
h cont
rols in every
treatment tested.
All the tested strains showed statis
ti
cally higher mortality when
compared to both controls, showing LC50 concentrations of
12.40
x
10
8
CFU
/mL
for
BTI193, 4.76
x
10
8
CFU
/mL
for
BTK176
and
4.16
x
10
8
CFU
/mL
for
BL102.
Transmission microg
raphs s
howed that every strain presented
cell damage profile
established for these species of entomopathogenic
bacteria, such as the high level of
cytoplasm vacuolization and dis
organization of ce
ll organelles, however, only
B.
thurin
giensis
strains showed microvilli deformation and disruption and cytoplasmic
content extruded into intestinal lumen.
All these observations suggest
that tested
B.
laterosporus
and
B. thuringiensis
strain
s
are
promising agent
s
to the biological
control
of
M. domestica
| Identifer | oai:union.ndltd.org:IBICT/oai:www.arca.fiocruz.br:icict/13355 |
| Date | January 2015 |
| Creators | Ferreira, Vítor dos Santos Baía |
| Contributors | Queiroz, Margareth Maria de Carvalho, d’Almeida, José Mario, Aguia, Valéria Magalhães, Braga, Marina Vianna, Costa, Gisela Lara da, Zahner, Viviane |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da FIOCRUZ, instname:Fundação Oswaldo Cruz, instacron:FIOCRUZ |
| Rights | info:eu-repo/semantics/openAccess |
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