Investigations into the phylogeny, genome size, and karyotype of microsporidian
Nadeispora canceri were initiated to further characterize the organism. Isolates of N.
canceri spores were obtained from both Dungeness (Cancer magisrer) and red rock crabs
(Cancer productus). Analysis of the ssu rDNA sequence from spore isolates of the two
crab species showed 100% sequence identity among 1,081 nucleotide positions,
indicating the same species of microsporidian is infecting both species of crabs.
Phylogenetic studies based on the ssu rDNA sequences also showed N. canceri to be
most closely related to another crustacean parasite, A meson michaelis. Sequence
comparison between the two microsporidian species showed 93% sequence identity
(1,001/1081 nucleotide positions).
Pulsed field gel electrophoresis was used to estimate the genome size and
karyotype of N. canceri isolates obtained from Dungeness and red rock crabs. Resolution
of DNA bands on the pulsed field gels revealed both isolates to have a karyotype often
chromosome-sized DNA bands. Estimation of the genome size revealed spore isolates
from C. magister to have a total genome size of 7.44 Mb and spore isolates from C.
productus to have a total genome size of 7.32 Mb. Variations detected in chromosome
size culminated in a difference in the genome size between the two isolates. However,
the variations in chromosome size were found not to be significant based on the Student's
t-test. / Graduation date: 2004
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/29856 |
| Date | 26 February 2004 |
| Creators | Amogan, Harold |
| Contributors | Reno, Paul W. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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