Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Dagmar Dolinská Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Work title: Advanced methods for testing antimicrobial activity Goal: The aim of the work in the theoretical part was to summarize methods of antimicrobial screening focusing on antimycotics and in the practical part to evaluate the antifungal activity of 47 tested substances using the microdilution broth method. Methods: The substances were tested using quantitative microdilution broth method on eight strains of yeast and fibrous fungi. These were Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis, Aspergillus fumigatus, Aspergillus flavus, Absidia corymbifera and Trichophyton interdigitale. Results: The highest antimycotic activity was demonstrated by compound Ac-0a, where we recorded after 24 hours of incubation activity against all eight strains. However, it was not able to stop the growth of three strains after 48 hours of incubation, the strains concerned were Candida parapsilosis, Aspergillus fumigatus and Aspergillus flavus. Other samples which have shown activity were Cl-2, where activity was observed for Candida krusei, Aspergillus fumigatus and Trichophyton interdigitale strains, Cl-3 for...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:387273 |
| Date | January 2018 |
| Creators | Dolinská, Dagmar |
| Contributors | Janďourek, Ondřej, Konečná, Klára |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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